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Assay Detail

CHEMBL667812

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to inhibit Lactobacillus casei dihydrofolate reductase in vitro was determined
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lacticaseibacillus casei
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL2902)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

Folate analogues altered in the C9-N10 bridge region. 16. Synthesis and antifolate activity of 11-thiohomoaminopterin.
Nair MG, Chen SY, Kisliuk RL, Gaumont Y, Strumpf D.
J Med Chem (1980) 23 : 899 -903
PubMed 6772788 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.21 8.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 8.02
CHEMBL10592 1 6.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE IC50 = 9.6 nM 8.02
CHEMBL10592 IC50 = 400.0 nM 6.40