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Assay Detail

CHEMBL667861

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Binding
Binding affinity against Dihydrofolate reductase of rat liver
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL2363)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Receptor-based design of dihydrofolate reductase inhibitors: comparison of crystallographically determined enzyme binding with enzyme affinity in a series of carboxy-substituted trimethoprim analogues.
Kuyper LF, Roth B, Baccanari DP, Ferone R, Beddell CR, Champness JN, Stammers DK, Dann JG, Norrington FE, Baker DJ.
J Med Chem (1985) 28 : 303 -311
PubMed 3973902 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 4.43 5.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL418347 1 5.01
CHEMBL13673 1 4.57
CHEMBL14001 1 4.46
CHEMBL274430 1 4.25
CHEMBL14201 1 4.16
CHEMBL13820 1 4.13
CHEMBL318078 1 -
CHEMBL321942 1 -
CHEMBL13796 1 -
CHEMBL13908 1 -
CHEMBL22 TRIMETHOPRIM 4.0 1 -
CHEMBL320596 1 -
CHEMBL13877 1 -
CHEMBL105006 1 -
CHEMBL14002 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL418347 IC50 = 9700.0 nM 5.01
CHEMBL13673 IC50 = 27000.0 nM 4.57
CHEMBL14001 IC50 = 35000.0 nM 4.46
CHEMBL274430 IC50 = 56000.0 nM 4.25
CHEMBL14201 IC50 = 70000.0 nM 4.16
CHEMBL13820 IC50 = 74000.0 nM 4.13
CHEMBL318078 IC50 = 1100000.0 nM -
CHEMBL321942 IC50 = 640000.0 nM -
CHEMBL13796 IC50 = 180000.0 nM -
CHEMBL13908 IC50 = 110000.0 nM -
CHEMBL22 TRIMETHOPRIM IC50 = 340000.0 nM -
CHEMBL320596 IC50 = 400000.0 nM -
CHEMBL13877 IC50 = 130000.0 nM -
CHEMBL105006 IC50 = 300000.0 nM -
CHEMBL14002 IC50 = 160000.0 nM -