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Assay Detail

CHEMBL667865

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat liver Dihydrofolate reductase.
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Dihydrofolate reductase (CHEMBL2363)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

2,4-Diamino-5-benzylpyrimidines and analogues as antibacterial agents. 5. 3',5'-Dimethoxy-4'-substituted-benzyl analogues of trimethoprim.
Roth B, Aig E, Rauckman BS, Strelitz JZ, Phillips AP, Ferone R, Bushby SR, Sigel CW.
J Med Chem (1981) 24 : 933 -941
PubMed 7035668 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 4.28 4.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL174091 1 4.82
CHEMBL279509 1 4.33
CHEMBL367061 1 4.30
CHEMBL172808 1 4.27
CHEMBL366869 1 4.16
CHEMBL287241 1 4.05
CHEMBL1181 4-HYDROXYTRIMETHOPRIM 1 4.04
CHEMBL105006 1 -
CHEMBL369211 1 -
CHEMBL318078 1 -
CHEMBL173901 1 -
CHEMBL176489 1 -
CHEMBL366561 1 -
CHEMBL173709 1 -
CHEMBL263732 1 -
CHEMBL369057 1 -
CHEMBL22 TRIMETHOPRIM 4.0 1 -
CHEMBL174309 1 -
CHEMBL174433 1 -
CHEMBL367218 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL174091 IC50 = 15000.0 nM 4.82
CHEMBL279509 IC50 = 47000.0 nM 4.33
CHEMBL367061 IC50 = 50000.0 nM 4.30
CHEMBL172808 IC50 = 54000.0 nM 4.27
CHEMBL366869 IC50 = 69000.0 nM 4.16
CHEMBL287241 IC50 = 90000.0 nM 4.05
CHEMBL1181 4-HYDROXYTRIMETHOPRIM IC50 = 92000.0 nM 4.04
CHEMBL105006 IC50 = 296000.0 nM -
CHEMBL369211 IC50 = 200000.0 nM -
CHEMBL318078 IC50 = 1100000.0 nM -
CHEMBL173901 IC50 = 580000.0 nM -
CHEMBL176489 IC50 = 250000.0 nM -
CHEMBL366561 IC50 > 440000.0 nM -
CHEMBL173709 IC50 > 400000.0 nM -
CHEMBL263732 IC50 = 120000.0 nM -
CHEMBL369057 IC50 = 560000.0 nM -
CHEMBL22 TRIMETHOPRIM IC50 = 260000.0 nM -
CHEMBL174309 IC50 = 270000.0 nM -
CHEMBL174433 IC50 = 200000.0 nM -
CHEMBL367218 IC50 = 600000.0 nM -