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Assay Detail

CHEMBL668120

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Binding
Inhibition against dihydrofolate reductase enzyme of Escherichia coli
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Escherichia coli
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dihydrofolate reductase (CHEMBL1809)
Type SINGLE PROTEIN
Organism Escherichia coli

Publication

2,4-Diamino-5-benzylpyrimidines as antibacterial agents. 4. 6-Substituted trimethoprim derivatives from phenolic Mannich intermediates. Application to the synthesis of trimethoprim and 3,5-dialkylbenzyl analogues.
Roth B, Aig E, Lane K, Rauckman BS.
J Med Chem (1980) 23 : 535 -541
PubMed 6991695 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.99 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL22 TRIMETHOPRIM 4.0 1 8.30
CHEMBL325533 1 7.00
CHEMBL339058 1 6.52
CHEMBL149353 1 6.10
CHEMBL150543 1 5.82
CHEMBL150248 1 5.70
CHEMBL357960 1 5.70
CHEMBL358879 1 5.52
CHEMBL149408 1 5.00
CHEMBL439995 1 4.22
CHEMBL150683 1 -
CHEMBL150699 1 -
CHEMBL150700 1 -
CHEMBL150017 1 -
CHEMBL150538 1 -
CHEMBL150863 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL22 TRIMETHOPRIM IC50 = 5.0 nM 8.30
CHEMBL325533 IC50 = 100.0 nM 7.00
CHEMBL339058 IC50 = 300.0 nM 6.52
CHEMBL149353 IC50 = 800.0 nM 6.10
CHEMBL150543 IC50 = 1500.0 nM 5.82
CHEMBL150248 IC50 = 2000.0 nM 5.70
CHEMBL357960 IC50 = 2000.0 nM 5.70
CHEMBL358879 IC50 = 3000.0 nM 5.52
CHEMBL149408 IC50 = 10000.0 nM 5.00
CHEMBL439995 IC50 = 60000.0 nM 4.22
CHEMBL150683 IC50 > 40000.0 nM -
CHEMBL150699 IC50 = - nM -
CHEMBL150700 IC50 > 450000.0 nM -
CHEMBL150017 IC50 = 4500000.0 nM -
CHEMBL150538 IC50 > 450000.0 nM -
CHEMBL150863 IC50 > 45000.0 nM -