Assay Detail
Binding
CHEMBL668878
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibitory activity against Dihydrofolate reductase enzyme purified from rat liver.
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
2,4-Diamino-5-benzylpyrimidines and analogues as antibacterial agents. 2. C-Alkylation of pyrimidines with Mannich bases and application to the synthesis of trimethoprim and analogues.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.04 | 4.04 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1181 | 4-HYDROXYTRIMETHOPRIM | — | 1 | 4.04 |
| CHEMBL325533 | — | — | 1 | - |
| CHEMBL22 | TRIMETHOPRIM | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1181 | 4-HYDROXYTRIMETHOPRIM | IC50 | = | 92000.0 | nM | 4.04 |
| CHEMBL325533 | — | IC50 | > | 400000.0 | nM | - |
| CHEMBL22 | TRIMETHOPRIM | IC50 | = | 260000.0 | nM | - |