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Assay Detail

CHEMBL669468

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro inhibitory concentration against beef liver dihydrofolate reductase
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL2902)
Type SINGLE PROTEIN
Organism Lactobacillus casei

Publication

Synthesis of the antileukemic agents 5,10-dideazaaminopterin and 5,10-dideaza-5,6,7,8-tetrahydroaminopterin.
Taylor EC, Harrington PJ, Fletcher SR, Beardsley GP, Moran RG.
J Med Chem (1985) 28 : 914 -921
PubMed 4009615 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.28 7.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL124631 1 7.37
CHEMBL173271 1 7.15
CHEMBL350097 1 4.31
CHEMBL170101 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL124631 IC50 = 43.0 nM 7.37
CHEMBL173271 IC50 = 71.0 nM 7.15
CHEMBL350097 IC50 = 49000.0 nM 4.31
CHEMBL170101 IC50 = 560000.0 nM -