Assay Detail
Binding
CHEMBL677127
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was tested for its ability to inhibit Folyl-polyglutamate synthase from mouse liver
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Folylpolyglutamate synthase, mitochondrial (CHEMBL2890) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Methotrexate analogues. 26. Inhibition of dihydrofolate reductase and folylpolyglutamate synthetase activity and in vitro tumor cell growth by methotrexate and aminopterin analogues containing a basic amino acid side chain.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 4 | 5.42 | 6.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL280864 | — | — | 1 | 6.82 |
| CHEMBL376180 | AMINOPTERIN | 2.0 | 1 | 4.75 |
| CHEMBL36083 | — | — | 1 | 4.69 |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL280864 | — | Ki | = | 150.0 | nM | 6.82 |
| CHEMBL376180 | AMINOPTERIN | Ki | = | 17600.0 | nM | 4.75 |
| CHEMBL36083 | — | Ki | = | 20400.0 | nM | 4.69 |
| CHEMBL34259 | METHOTREXATE | Ki | = | 166000.0 | nM | - |