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Assay Detail

CHEMBL679948

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGF-stimulated autophosphorylation of EGFR in A431 cells
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A-431
Confidence 9 — Direct single protein target
Curated By Expert

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tyrosine kinase inhibitors. 18. 6-Substituted 4-anilinoquinazolines and 4-anilinopyrido[3,4-d]pyrimidines as soluble, irreversible inhibitors of the epidermal growth factor receptor.
Smaill JB, Showalter HD, Zhou H, Bridges AJ, McNamara DJ, Fry DW, Nelson JM, Sherwood V, Vincent PW, Roberts BJ, Elliott WL, Denny WA.
J Med Chem (2001) 44 : 429 -440
PubMed 11462982 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 7.28 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL434827 1 7.68
CHEMBL334801 1 7.24
CHEMBL345109 1 7.23
CHEMBL140561 1 6.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL434827 IC50 = 21.0 nM 7.68
CHEMBL334801 IC50 = 57.0 nM 7.24
CHEMBL345109 IC50 = 59.0 nM 7.23
CHEMBL140561 IC50 = 108.0 nM 6.97