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Assay Detail

CHEMBL680372

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro glycine reuptake inhibitory activity against glycine transporter type-2 (GlyT-2) transporter cells after incubation at 30 degrees for 30 min in presence of 50 nM [3H]glycine.
29
Total Activities
29
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Sodium- and chloride-dependent glycine transporter 2 (CHEMBL3195)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

5,5-Diaryl-2-amino-4-pentenoates as novel, potent, and selective glycine transporter type-2 reuptake inhibitors.
Isaac M, Slassi A, Silva KD, Arora J, MacLean N, Hung B, McCallum K.
Bioorg Med Chem Lett (2001) 11 : 1371 -1373
PubMed 11378357 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 29 5.90 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL28578 1 6.82
CHEMBL29608 1 6.55
CHEMBL29220 1 6.54
CHEMBL28746 1 6.48
CHEMBL30144 1 6.44
CHEMBL29958 1 6.40
CHEMBL28601 1 6.34
CHEMBL418388 1 6.18
CHEMBL28577 1 6.11
CHEMBL285370 1 5.99
CHEMBL29964 1 5.94
CHEMBL29023 1 5.93
CHEMBL27914 1 5.87
CHEMBL28549 1 5.80
CHEMBL283230 1 5.76
CHEMBL29279 1 5.69
CHEMBL281450 1 5.69
CHEMBL283017 1 5.69
CHEMBL27850 1 5.62
CHEMBL285592 1 5.58
CHEMBL29022 1 5.56
CHEMBL29396 1 5.44
CHEMBL286706 1 5.26
CHEMBL28570 1 5.23
CHEMBL28016 1 5.23
CHEMBL29256 1 5.19
CHEMBL416203 1 -
CHEMBL1159913 1 -
CHEMBL287626 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL28578 IC50 = 150.0 nM 6.82
CHEMBL29608 IC50 = 283.0 nM 6.55
CHEMBL29220 IC50 = 290.0 nM 6.54
CHEMBL28746 IC50 = 330.0 nM 6.48
CHEMBL30144 IC50 = 365.0 nM 6.44
CHEMBL29958 IC50 = 400.0 nM 6.40
CHEMBL28601 IC50 = 460.0 nM 6.34
CHEMBL418388 IC50 = 660.0 nM 6.18
CHEMBL28577 IC50 = 770.0 nM 6.11
CHEMBL285370 IC50 = 1030.0 nM 5.99
CHEMBL29964 IC50 = 1160.0 nM 5.94
CHEMBL29023 IC50 = 1170.0 nM 5.93
CHEMBL27914 IC50 = 1340.0 nM 5.87
CHEMBL28549 IC50 = 1590.0 nM 5.80
CHEMBL283230 IC50 = 1740.0 nM 5.76
CHEMBL29279 IC50 = 2030.0 nM 5.69
CHEMBL281450 IC50 = 2060.0 nM 5.69
CHEMBL283017 IC50 = 2030.0 nM 5.69
CHEMBL27850 IC50 = 2400.0 nM 5.62
CHEMBL285592 IC50 = 2640.0 nM 5.58
CHEMBL29022 IC50 = 2750.0 nM 5.56
CHEMBL29396 IC50 = 3660.0 nM 5.44
CHEMBL286706 IC50 = 5470.0 nM 5.26
CHEMBL28570 IC50 = 5930.0 nM 5.23
CHEMBL28016 IC50 = 5880.0 nM 5.23
CHEMBL29256 IC50 = 6440.0 nM 5.19
CHEMBL416203 IC50 > 10000.0 nM -
CHEMBL1159913 IC50 > 10000.0 nM -
CHEMBL287626 IC50 > 10000.0 nM -