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Assay Detail

CHEMBL680647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity against benzodiazepine site of gamma-aminobutyric-acid A receptor of rat cerebral cortex by using [3H]flunitrazepam in the presence of GABA 1 mM.
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Cerebral cortex
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Potential anxiolytic agents. Pyrido[1,2-a]benzimidazoles: a new structural class of ligands for the benzodiazepine binding site on GABA-A receptors.
Maryanoff BE, Ho W, McComsey DF, Reitz AB, Grous PP, Nortey SO, Shank RP, Dubinsky B, Taylor RJ, Gardocki JF.
J Med Chem (1995) 38 : 16 -20
PubMed 7837226 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 7.69 9.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL413744 1 9.96
CHEMBL59764 1 9.59
CHEMBL64169 1 8.85
CHEMBL64768 1 8.85
CHEMBL292583 1 8.77
CHEMBL303784 1 8.68
CHEMBL304464 1 8.57
CHEMBL294727 1 8.28
CHEMBL2052026 1 8.23
CHEMBL12 DIAZEPAM 4.0 1 7.89
CHEMBL64225 1 7.85
CHEMBL63832 1 7.80
CHEMBL60668 1 7.80
CHEMBL292675 1 7.75
CHEMBL292651 1 7.60
CHEMBL292595 1 7.58
CHEMBL304012 1 7.14
CHEMBL64106 1 7.02
CHEMBL64637 1 6.82
CHEMBL60127 1 6.77
CHEMBL432298 1 6.58
CHEMBL416318 1 6.50
CHEMBL302420 1 6.08
CHEMBL58181 1 6.03
CHEMBL58120 1 5.22
CHEMBL60466 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL413744 IC50 = 0.11 nM 9.96
CHEMBL59764 IC50 = 0.26 nM 9.59
CHEMBL64169 IC50 = 1.4 nM 8.85
CHEMBL64768 IC50 = 1.4 nM 8.85
CHEMBL292583 IC50 = 1.7 nM 8.77
CHEMBL303784 IC50 = 2.1 nM 8.68
CHEMBL304464 IC50 = 2.7 nM 8.57
CHEMBL294727 IC50 = 5.3 nM 8.28
CHEMBL2052026 IC50 = 5.9 nM 8.23
CHEMBL12 DIAZEPAM IC50 = 13.0 nM 7.89
CHEMBL64225 IC50 = 14.0 nM 7.85
CHEMBL63832 IC50 = 16.0 nM 7.80
CHEMBL60668 IC50 = 16.0 nM 7.80
CHEMBL292675 IC50 = 18.0 nM 7.75
CHEMBL292651 IC50 = 25.0 nM 7.60
CHEMBL292595 IC50 = 26.0 nM 7.58
CHEMBL304012 IC50 = 72.0 nM 7.14
CHEMBL64106 IC50 = 95.0 nM 7.02
CHEMBL64637 IC50 = 150.0 nM 6.82
CHEMBL60127 IC50 = 170.0 nM 6.77
CHEMBL432298 IC50 = 265.0 nM 6.58
CHEMBL416318 IC50 = 320.0 nM 6.50
CHEMBL302420 IC50 = 840.0 nM 6.08
CHEMBL58181 IC50 = 940.0 nM 6.03
CHEMBL58120 IC50 = 6000.0 nM 5.22
CHEMBL60466 IC50 > 10000.0 nM -