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Assay Detail

CHEMBL681273

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of binding of tert-butyl bicyclophosphorothionate ([35 S]TBPS) at Gamma-aminobutyric acid A receptor from rat whole brain membranes
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Subcellular Membrane
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Conformationally constrained anesthetic steroids that modulate GABA(A) receptors.
Anderson A, Boyd AC, Clark JK, Fielding L, Gemmell DK, Hamilton NM, Maidment MS, May V, McGuire R, McPhail P, Sansbury FH, Sundaram H, Taylor R.
J Med Chem (2000) 43 : 4118 -4125
PubMed 11063608 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.34 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL429756 1 7.26
CHEMBL210952 ELTANOLONE 2.0 1 6.84
CHEMBL207538 BREXANOLONE 4.0 1 6.81
CHEMBL133736 1 6.80
CHEMBL1256760 1 6.72
CHEMBL422984 1 6.68
CHEMBL510813 1 6.66
CHEMBL130165 1 6.63
CHEMBL337881 1 6.59
CHEMBL190279 ALFAXALONE 2.0 1 6.37
CHEMBL341185 1 5.91
CHEMBL87285 ANDROSTERONE 1 5.86
CHEMBL422795 1 5.75
CHEMBL335062 ANDROSTANDIOL 1 5.73
CHEMBL334621 1 5.60
CHEMBL336207 1 5.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL429756 IC50 = 55.0 nM 7.26
CHEMBL210952 ELTANOLONE IC50 = 145.0 nM 6.84
CHEMBL207538 BREXANOLONE IC50 = 155.0 nM 6.81
CHEMBL133736 IC50 = 159.0 nM 6.80
CHEMBL1256760 IC50 = 192.0 nM 6.72
CHEMBL422984 IC50 = 210.0 nM 6.68
CHEMBL510813 IC50 = 221.0 nM 6.66
CHEMBL130165 IC50 = 232.0 nM 6.63
CHEMBL337881 IC50 = 255.0 nM 6.59
CHEMBL190279 ALFAXALONE IC50 = 423.0 nM 6.37
CHEMBL341185 IC50 = 1236.0 nM 5.91
CHEMBL87285 ANDROSTERONE IC50 = 1367.0 nM 5.86
CHEMBL422795 IC50 = 1767.0 nM 5.75
CHEMBL335062 ANDROSTANDIOL IC50 = 1867.0 nM 5.73
CHEMBL334621 IC50 = 2533.0 nM 5.60
CHEMBL336207 IC50 = 5350.0 nM 5.27