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Assay Detail

CHEMBL681298

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro ability to displace radiolabeled FTI from farnesyltransferase in cultured Ha-ras transformed RAT1 cells
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type Rat1
Confidence 6 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein farnesyltransferase (CHEMBL2095197)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Evaluation of amino acid-based linkers in potent macrocyclic inhibitors of farnesyl-protein transferase.
Beshore DC, Bell IM, Dinsmore CJ, Homnick CF, Culberson JC, Robinson RG, Fernandes C, Walsh ES, Abrams MT, Bhimnathwala HG, Davide JP, Ellis-Hutchings MS, Huber HA, Koblan KS, Buser CA, Kohl NE, Lobell RB, Chen IW, McLoughlin DA, Olah TV, Graham SL, Hartman GD, Williams TM.
Bioorg Med Chem Lett (2001) 11 : 1817 -1821
PubMed 11459639 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 7.07 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL54212 1 8.15
CHEMBL53069 1 7.89
CHEMBL418268 1 7.82
CHEMBL299568 1 7.77
CHEMBL54043 1 7.77
CHEMBL298895 1 7.64
CHEMBL298392 1 7.53
CHEMBL52975 1 7.43
CHEMBL52921 1 7.37
CHEMBL53183 1 7.26
CHEMBL50857 1 7.19
CHEMBL53811 1 7.17
CHEMBL53037 1 7.00
CHEMBL53862 1 6.74
CHEMBL301491 1 6.71
CHEMBL300966 1 6.62
CHEMBL298337 1 6.61
CHEMBL53154 1 6.59
CHEMBL52610 1 6.51
CHEMBL423303 1 6.39
CHEMBL300206 1 6.39
CHEMBL299621 1 6.24
CHEMBL52859 1 5.88
CHEMBL54969 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL54212 IC50 = 7.1 nM 8.15
CHEMBL53069 IC50 = 13.0 nM 7.89
CHEMBL418268 IC50 = 15.0 nM 7.82
CHEMBL299568 IC50 = 17.0 nM 7.77
CHEMBL54043 IC50 = 17.0 nM 7.77
CHEMBL298895 IC50 = 23.0 nM 7.64
CHEMBL298392 IC50 = 29.6 nM 7.53
CHEMBL52975 IC50 = 37.0 nM 7.43
CHEMBL52921 IC50 = 43.0 nM 7.37
CHEMBL53183 IC50 = 55.0 nM 7.26
CHEMBL50857 IC50 = 65.0 nM 7.19
CHEMBL53811 IC50 = 67.0 nM 7.17
CHEMBL53037 IC50 = 99.0 nM 7.00
CHEMBL53862 IC50 = 184.0 nM 6.74
CHEMBL301491 IC50 = 196.0 nM 6.71
CHEMBL300966 IC50 = 240.0 nM 6.62
CHEMBL298337 IC50 = 248.0 nM 6.61
CHEMBL53154 IC50 = 258.0 nM 6.59
CHEMBL52610 IC50 = 310.0 nM 6.51
CHEMBL423303 IC50 = 410.0 nM 6.39
CHEMBL300206 IC50 = 409.0 nM 6.39
CHEMBL299621 IC50 = 574.0 nM 6.24
CHEMBL52859 IC50 = 1310.0 nM 5.88
CHEMBL54969 IC50 > 100.0 nM -