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Assay Detail

CHEMBL681945

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Non-competitive displacement of [35S]TBPS from picrotoxin binding site found in heterogeneous Gamma-aminobutyric acid A receptor in rat brain membranes.
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Brain membranes
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Neurosteroid analogues. 9. Conformationally constrained pregnanes: structure-activity studies of 13,24-cyclo-18,21-dinorcholane analogues of the GABA modulatory and anesthetic steroids (3alpha,5alpha)- and (3alpha,5beta)-3-hydroxypregnan-20-one.
Jiang X, Manion BD, Benz A, Rath NP, Evers AS, Zorumski CF, Mennerick S, Covey DF.
J Med Chem (2003) 46 : 5334 -5348
PubMed 14640542 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.26 7.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL210952 ELTANOLONE 2.0 1 7.15
CHEMBL3137969 1 6.98
CHEMBL3137966 1 6.71
CHEMBL3137971 1 6.61
CHEMBL3137962 1 6.57
CHEMBL3137960 1 6.52
CHEMBL3137961 1 6.48
CHEMBL3137959 1 6.48
CHEMBL3138304 1 6.43
CHEMBL3138306 1 6.29
CHEMBL3138273 1 6.05
CHEMBL3137970 1 5.90
CHEMBL3138399 1 5.84
CHEMBL3137958 1 5.80
CHEMBL3138305 1 5.75
CHEMBL3138309 1 5.49
CHEMBL3138269 1 5.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL210952 ELTANOLONE IC50 = 71.0 nM 7.15
CHEMBL3137969 IC50 = 105.0 nM 6.98
CHEMBL3137966 IC50 = 197.0 nM 6.71
CHEMBL3137971 IC50 = 243.0 nM 6.61
CHEMBL3137962 IC50 = 268.0 nM 6.57
CHEMBL3137960 IC50 = 301.0 nM 6.52
CHEMBL3137961 IC50 = 334.0 nM 6.48
CHEMBL3137959 IC50 = 329.0 nM 6.48
CHEMBL3138304 IC50 = 373.0 nM 6.43
CHEMBL3138306 IC50 = 514.0 nM 6.29
CHEMBL3138273 IC50 = 899.0 nM 6.05
CHEMBL3137970 IC50 = 1260.0 nM 5.90
CHEMBL3138399 IC50 = 1440.0 nM 5.84
CHEMBL3137958 IC50 = 1570.0 nM 5.80
CHEMBL3138305 IC50 = 1780.0 nM 5.75
CHEMBL3138309 IC50 = 3230.0 nM 5.49
CHEMBL3138269 IC50 = 4750.0 nM 5.32