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Assay Detail

CHEMBL681947

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3S]-tert-butylbicyclophosphorothionate (TBPS) from Gamma-aminobutyric acid A (GABA-A) receptor of rat brain membranes
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

GABA-A receptor; anion channel (CHEMBL1907607)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Neurosteroid analogues. 8. Structure-activity studies of N-acylated 17a-aza-D-homosteroid analogues of the anesthetic steroids (3alpha, 5alpha)- and (3alpha,5beta)-3-hydroxypregnan-20-one.
Covey DF, Han M, Kumar AS, de La Cruz MA, Meadows ES, Hu Y, Tonnies A, Nathan D, Coleman M, Benz A, Evers AS, Zorumski CF, Mennerick S.
J Med Chem (2000) 43 : 3201 -3204
PubMed 10966737 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.46 7.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1256760 1 7.41
CHEMBL108614 1 7.35
CHEMBL210952 ELTANOLONE 2.0 1 7.15
CHEMBL207538 BREXANOLONE 4.0 1 7.13
CHEMBL3140372 1 6.92
CHEMBL107932 1 6.70
CHEMBL111792 1 6.37
CHEMBL325363 1 6.26
CHEMBL107980 1 6.03
CHEMBL421415 1 5.58
CHEMBL432604 1 5.46
CHEMBL321866 1 5.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1256760 IC50 = 39.0 nM 7.41
CHEMBL108614 IC50 = 45.0 nM 7.35
CHEMBL210952 ELTANOLONE IC50 = 71.0 nM 7.15
CHEMBL207538 BREXANOLONE IC50 = 74.0 nM 7.13
CHEMBL3140372 IC50 = 120.0 nM 6.92
CHEMBL107932 IC50 = 200.0 nM 6.70
CHEMBL111792 IC50 = 430.0 nM 6.37
CHEMBL325363 IC50 = 550.0 nM 6.26
CHEMBL107980 IC50 = 930.0 nM 6.03
CHEMBL421415 IC50 = 2610.0 nM 5.58
CHEMBL432604 IC50 = 3470.0 nM 5.46
CHEMBL321866 IC50 = 6220.0 nM 5.21