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Assay Detail

CHEMBL687657

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of purified H+/K+ ATPase at pH 7.4 as released inorganic phosphate from ATP using hog stomach gastric membrane vesicles
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Tissue Stomach
Confidence 7 — Homologous single protein target
Curated By Intermediate

Target

Potassium-transporting ATPase (CHEMBL2095228)
Type PROTEIN COMPLEX
Organism Sus scrofa

Publication

Antiulcer agents. 6. Analysis of the in vitro biochemical and in vivo gastric antisecretory activity of substituted imidazo[1,2-a]pyridines and related analogues using comparative molecular field analysis and hypothetical active site lattice methodologies.
Kaminski JJ, Doweyko AM.
J Med Chem (1997) 40 : 427 -436
PubMed 9046332 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.55 7.04

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL296482 1 7.04
CHEMBL64216 1 6.05
CHEMBL47529 1 5.80
CHEMBL296417 1 5.78
CHEMBL293244 1 5.70
CHEMBL296544 1 5.68
CHEMBL137379 1 5.66
CHEMBL137700 1 5.07
CHEMBL43945 1 4.86
CHEMBL137742 1 4.85
CHEMBL45835 1 4.55
CHEMBL136140 1 -
CHEMBL46595 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL296482 IC50 = 92.0 nM 7.04
CHEMBL64216 IC50 = 890.0 nM 6.05
CHEMBL47529 IC50 = 1590.0 nM 5.80
CHEMBL296417 IC50 = 1680.0 nM 5.78
CHEMBL293244 IC50 = 2000.0 nM 5.70
CHEMBL296544 IC50 = 2100.0 nM 5.68
CHEMBL137379 IC50 = 2200.0 nM 5.66
CHEMBL137700 IC50 = 8600.0 nM 5.07
CHEMBL43945 IC50 = 13700.0 nM 4.86
CHEMBL137742 IC50 = 14000.0 nM 4.85
CHEMBL45835 IC50 = 28400.0 nM 4.55
CHEMBL136140 IC50 = 206000.0 nM -
CHEMBL46595 IC50 = 178000.0 nM -