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Assay Detail

CHEMBL689908

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Concentration resulting in 50% incorporation of [3H]thymidine compared to untreated control cells on H2981 human lung adenocarcinoma cells in the presence of 10 ug/mL Enterobacter cloacae P99 beta-lactamase (ECIbL)
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type H2981
Confidence 1 — Organism
Curated By Intermediate

Target

H2981 cell line (CHEMBL614802)
Type CELL-LINE
Organism Homo sapiens

Publication

Cephalosporin derivatives of doxorubicin as prodrugs for activation by monoclonal antibody-beta-lactamase conjugates.
Vrudhula VM, Svensson HP, Senter PD.
J Med Chem (1995) 38 : 1380 -1385
PubMed 7731023 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.29 6.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL28740 1 6.37
CHEMBL263250 1 6.35
CHEMBL406975 1 6.33
CHEMBL282534 1 6.33
CHEMBL283005 1 6.26
CHEMBL282621 1 6.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL28740 IC50 = 430.0 nM 6.37
CHEMBL263250 IC50 = 450.0 nM 6.35
CHEMBL406975 IC50 = 470.0 nM 6.33
CHEMBL282534 IC50 = 470.0 nM 6.33
CHEMBL283005 IC50 = 550.0 nM 6.26
CHEMBL282621 IC50 = 750.0 nM 6.12