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Assay Detail

CHEMBL694352

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro cholesterol biosynthesis inhibition in human HepG2 by measuring the extend of cholesterol radiolabeling after incubation with [14C]mevalonate.
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HepG2
Confidence 1 — Organism
Curated By Intermediate

Target

HepG2 (CHEMBL395)
Type CELL-LINE
Organism Homo sapiens

Publication

Design and synthesis of new potential photoaffinity labels for mammalian squalene epoxidase
Gotteland J, Dax C, Halazy S
Bioorg Med Chem Lett (1997) 7 : 1153 -1156
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.45 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL15929 1 7.22
CHEMBL16178 1 7.16
CHEMBL267332 1 6.27
CHEMBL16004 1 5.92
CHEMBL16120 1 5.68
CHEMBL15811 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL15929 IC50 = 60.0 nM 7.22
CHEMBL16178 IC50 = 70.0 nM 7.16
CHEMBL267332 IC50 = 540.0 nM 6.27
CHEMBL16004 IC50 = 1200.0 nM 5.92
CHEMBL16120 IC50 = 2100.0 nM 5.68
CHEMBL15811 IC50 > 100000.0 nM -