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Assay Detail

CHEMBL694892

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Binding
Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H3 receptor
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type SK-N-MC
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of conformationally constrained histamine H(3) receptor agonists.
Kitbunnadaj R, Zuiderveld OP, De Esch IJ, Vollinga RC, Bakker R, Lutz M, Spek AL, Cavoy E, Deltent MF, Menge WM, Timmerman H, Leurs R.
J Med Chem (2003) 46 : 5445 -5457
PubMed 14640553 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 7.66 9.88

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18661 IMMEPIP 1 9.88
CHEMBL153051 1 9.41
CHEMBL268229 1 9.17
CHEMBL153061 1 8.88
CHEMBL440730 1 8.68
CHEMBL152936 1 8.63
CHEMBL417096 IMPENTAMINE 1 8.63
CHEMBL356666 1 8.50
CHEMBL13795 VUF-5297 1 8.23
CHEMBL90019 IMBUTAMINE 1 8.13
CHEMBL78498 1 8.08
CHEMBL90 HISTAMINE 4.0 1 8.01
CHEMBL150701 1 7.88
CHEMBL153284 1 7.71
CHEMBL554031 1 7.48
CHEMBL91353 HOMOHISTAMINE 1 7.37
CHEMBL151467 1 7.31
CHEMBL13559 VUF-5296 1 7.24
CHEMBL152948 1 6.49
CHEMBL153207 1 6.29
CHEMBL154529 1 6.29
CHEMBL150703 1 6.10
CHEMBL150243 1 5.82
CHEMBL358028 1 5.74
CHEMBL345954 1 5.56
CHEMBL150512 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18661 IMMEPIP EC50 = 0.1318 nM 9.88
CHEMBL153051 EC50 = 0.389 nM 9.41
CHEMBL268229 EC50 = 0.6761 nM 9.17
CHEMBL153061 EC50 = 1.318 nM 8.88
CHEMBL440730 EC50 = 2.089 nM 8.68
CHEMBL152936 EC50 = 2.344 nM 8.63
CHEMBL417096 IMPENTAMINE EC50 = 2.344 nM 8.63
CHEMBL356666 EC50 = 3.162 nM 8.50
CHEMBL13795 VUF-5297 EC50 = 5.888 nM 8.23
CHEMBL90019 IMBUTAMINE EC50 = 7.413 nM 8.13
CHEMBL78498 EC50 = 8.318 nM 8.08
CHEMBL90 HISTAMINE EC50 = 9.772 nM 8.01
CHEMBL150701 EC50 = 13.18 nM 7.88
CHEMBL153284 EC50 = 19.5 nM 7.71
CHEMBL554031 EC50 = 33.11 nM 7.48
CHEMBL91353 HOMOHISTAMINE EC50 = 42.66 nM 7.37
CHEMBL151467 EC50 = 48.98 nM 7.31
CHEMBL13559 VUF-5296 EC50 = 57.54 nM 7.24
CHEMBL152948 EC50 = 323.59 nM 6.49
CHEMBL153207 EC50 = 512.86 nM 6.29
CHEMBL154529 EC50 = 512.86 nM 6.29
CHEMBL150703 EC50 = 794.33 nM 6.10
CHEMBL150243 EC50 = 1513.56 nM 5.82
CHEMBL358028 EC50 = 1819.7 nM 5.74
CHEMBL345954 EC50 = 2754.23 nM 5.56
CHEMBL150512 EC50 < 10000.0 nM -