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Assay Detail

CHEMBL696974

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]AMPA from human Ionotropic glutamate receptor AMPA 2 expressed in HEK293 cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Glutamate receptor 2 (CHEMBL4016)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of willardiine and 6-azawillardiine analogs: pharmacological characterization on cloned homomeric human AMPA and kainate receptor subtypes.
Jane DE, Hoo K, Kamboj R, Deverill M, Bleakman D, Mandelzys A.
J Med Chem (1997) 40 : 3645 -3650
PubMed 9357531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 6.77 7.78

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL331696 1 7.78
CHEMBL331644 1 7.71
CHEMBL123132 1 7.60
CHEMBL121388 1 7.28
CHEMBL121553 1 7.00
CHEMBL333964 1 7.00
CHEMBL276815 (S)-AMPA 1 6.97
CHEMBL121191 1 6.94
CHEMBL121915 1 6.75
CHEMBL120808 1 6.43
CHEMBL122656 1 6.34
CHEMBL122005 (S)-WILLARDIINE 1 6.05
CHEMBL575060 GLUTAMIC ACID 3.0 1 6.03
CHEMBL275040 KAINIC ACID 2.0 1 4.91

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL331696 Ki = 16.7 nM 7.78
CHEMBL331644 Ki = 19.3 nM 7.71
CHEMBL123132 Ki = 25.1 nM 7.60
CHEMBL121388 Ki = 53.1 nM 7.28
CHEMBL121553 Ki = 101.0 nM 7.00
CHEMBL333964 Ki = 101.0 nM 7.00
CHEMBL276815 (S)-AMPA Ki = 107.0 nM 6.97
CHEMBL121191 Ki = 115.0 nM 6.94
CHEMBL121915 Ki = 176.0 nM 6.75
CHEMBL120808 Ki = 372.0 nM 6.43
CHEMBL122656 Ki = 461.0 nM 6.34
CHEMBL122005 (S)-WILLARDIINE Ki = 898.0 nM 6.05
CHEMBL575060 GLUTAMIC ACID Ki = 940.0 nM 6.03
CHEMBL275040 KAINIC ACID Ki = 12200.0 nM 4.91