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Assay Detail

CHEMBL697561

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of growth (cytotoxicity) of HuTu 80 cell line in vitro
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HuTu80
Confidence 1 — Organism
Curated By Intermediate

Target

HuTu80 (CHEMBL614582)
Type CELL-LINE
Organism Homo sapiens

Publication

Novel fluorinated antifolates. Enzyme inhibition and cytotoxicity studies on 2'- and 3'-fluoroaminopterin.
Henkin J, Washtien WL.
J Med Chem (1983) 26 : 1193 -1196
PubMed 6410066 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 8.41 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18925 1 8.64
CHEMBL34259 METHOTREXATE 4.0 1 8.35
CHEMBL376180 AMINOPTERIN 2.0 1 8.33
CHEMBL19227 1 8.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18925 EC50 = 2.3 nM 8.64
CHEMBL34259 METHOTREXATE EC50 = 4.5 nM 8.35
CHEMBL376180 AMINOPTERIN EC50 = 4.7 nM 8.33
CHEMBL19227 EC50 = 4.8 nM 8.32