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Assay Detail

CHEMBL698076

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]kainate from human Ionotropic glutamate receptor ionotropic kainate 1 expressed in HEK293 cells
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Glutamate receptor ionotropic, kainate 5 (CHEMBL2675)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of willardiine and 6-azawillardiine analogs: pharmacological characterization on cloned homomeric human AMPA and kainate receptor subtypes.
Jane DE, Hoo K, Kamboj R, Deverill M, Bleakman D, Mandelzys A.
J Med Chem (1997) 40 : 3645 -3650
PubMed 9357531 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 14 6.83 9.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL121915 1 9.62
CHEMBL331644 1 8.54
CHEMBL333964 1 8.04
CHEMBL121553 1 7.70
CHEMBL331696 1 7.51
CHEMBL121388 1 7.24
CHEMBL275040 KAINIC ACID 2.0 1 6.75
CHEMBL121191 1 6.41
CHEMBL575060 GLUTAMIC ACID 3.0 1 6.15
CHEMBL120808 1 5.94
CHEMBL123132 1 5.74
CHEMBL122656 1 5.70
CHEMBL276815 (S)-AMPA 1 5.67
CHEMBL122005 (S)-WILLARDIINE 1 4.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL121915 Ki = 0.24 nM 9.62
CHEMBL331644 Ki = 2.9 nM 8.54
CHEMBL333964 Ki = 9.1 nM 8.04
CHEMBL121553 Ki = 19.8 nM 7.70
CHEMBL331696 Ki = 30.9 nM 7.51
CHEMBL121388 Ki = 57.1 nM 7.24
CHEMBL275040 KAINIC ACID Ki = 177.0 nM 6.75
CHEMBL121191 Ki = 393.0 nM 6.41
CHEMBL575060 GLUTAMIC ACID Ki = 701.0 nM 6.15
CHEMBL120808 Ki = 1150.0 nM 5.94
CHEMBL123132 Ki = 1820.0 nM 5.74
CHEMBL122656 Ki = 1980.0 nM 5.70
CHEMBL276815 (S)-AMPA Ki = 2144.0 nM 5.67
CHEMBL122005 (S)-WILLARDIINE Ki = 28900.0 nM 4.54