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Assay Detail

CHEMBL710408

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
In vitro antagonist effect on estrogen receptor alpha transcriptional activation in MCF-7 cells against 10 pM 17-beta-estradiol
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MCF7
Confidence 9 — Direct single protein target
Curated By Expert

Target

Estrogen receptor (CHEMBL206)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design, synthesis, and preclinical characterization of novel, highly selective indole estrogens.
Miller CP, Collini MD, Tran BD, Harris HA, Kharode YP, Marzolf JT, Moran RA, Henderson RA, Bender RH, Unwalla RJ, Greenberger LM, Yardley JP, Abou-Gharbia MA, Lyttle CR, Komm BS.
J Med Chem (2001) 44 : 1654 -1657
PubMed 11356100 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 8.39 9.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL81 RALOXIFENE 4.0 1 9.14
CHEMBL44426 PIPENDOXIFENE 2.0 1 8.82
CHEMBL46740 BAZEDOXIFENE 4.0 1 8.43
CHEMBL46903 1 7.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL81 RALOXIFENE IC50 = 0.72 nM 9.14
CHEMBL44426 PIPENDOXIFENE IC50 = 1.5 nM 8.82
CHEMBL46740 BAZEDOXIFENE IC50 = 3.7 nM 8.43
CHEMBL46903 IC50 = 69.0 nM 7.16