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Assay Detail

CHEMBL741875

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]- quinuclidinyl benzilate binding to Muscarinic acetylcholine receptor of rat cerebral cortical membranes
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL1907609)
Type PROTEIN FAMILY
Organism Rattus norvegicus

Publication

6beta-Acyloxy(nor)tropanes: affinities for antagonist/agonist binding sites on transfected and native muscarinic receptors.
Daly JW, Gupta TH, Padgett WL, Pei XF.
J Med Chem (2000) 43 : 2514 -2522
PubMed 10891110 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 4.86 6.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL84113 1 6.10
CHEMBL85190 1 5.75
CHEMBL315428 1 5.66
CHEMBL313738 1 5.07
CHEMBL314493 1 4.64
CHEMBL82754 1 4.60
CHEMBL315630 1 4.52
CHEMBL315749 1 4.51
CHEMBL310558 1 4.50
CHEMBL316443 1 4.47
CHEMBL408847 1 4.28
CHEMBL7634 OXOTREMORINE 1 4.24
CHEMBL294273 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL84113 Ki = 800.0 nM 6.10
CHEMBL85190 Ki = 1800.0 nM 5.75
CHEMBL315428 Ki = 2200.0 nM 5.66
CHEMBL313738 Ki = 8500.0 nM 5.07
CHEMBL314493 Ki = 23000.0 nM 4.64
CHEMBL82754 Ki = 25000.0 nM 4.60
CHEMBL315630 Ki = 30000.0 nM 4.52
CHEMBL315749 Ki = 31000.0 nM 4.51
CHEMBL310558 Ki = 32000.0 nM 4.50
CHEMBL316443 Ki = 34000.0 nM 4.47
CHEMBL408847 Ki = 53000.0 nM 4.28
CHEMBL7634 OXOTREMORINE Ki = 58000.0 nM 4.24
CHEMBL294273 Ki = 280000.0 nM -