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Assay Detail

CHEMBL744481

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Compound was evaluated for the competitive inhibition of [3H]methylscopolamine binding to muscarinic acetylcholine receptor M2 of mouse cerebral cortex
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Tissue Cerebral cortex
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL3197)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Synthesis and cholinergic properties of bis[[(dimethylamino)methyl]furanyl] analogues of ranitidine.
Sowell JW, Tang Y, Valli MJ, Chapman JM, Usher LA, Vaughan CM, Kosh JW.
J Med Chem (1992) 35 : 1102 -1108
PubMed 1552502 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.27 6.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL418712 1 6.40
CHEMBL13206 1 6.30
CHEMBL418714 1 6.27
CHEMBL13122 1 6.23
CHEMBL13426 1 6.15
CHEMBL94 PHYSOSTIGMINE 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL418712 IC50 = 400.0 nM 6.40
CHEMBL13206 IC50 = 500.0 nM 6.30
CHEMBL418714 IC50 = 540.0 nM 6.27
CHEMBL13122 IC50 = 590.0 nM 6.23
CHEMBL13426 IC50 = 710.0 nM 6.15
CHEMBL94 PHYSOSTIGMINE IC50 = 160000.0 nM -