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Assay Detail

CHEMBL745664

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to displace [3H]pirenzepine radioligand for the Muscarinic acetylcholine receptor M1 in rat brain
9
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Enantioselective synthesis of S-(+)-2beta-carboalkoxy-3alpha-[bis(4-fluorophenyl)methoxy]tropanes as novel probes for the dopamine transporter.
Zou MF, Agoston GE, Belov Y, Kopajtic T, Katz JL, Newman AH.
Bioorg Med Chem Lett (2002) 12 : 1249 -1252
PubMed 11965364 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 6.01 7.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL317757 DIFLUOROBENZTROPINE 1 7.94
CHEMBL21859 2 6.88
CHEMBL281388 2 5.73
CHEMBL428704 2 5.57
CHEMBL283154 2 5.36

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL317757 DIFLUOROBENZTROPINE Ki = 11.6 nM 7.94
CHEMBL21859 Ki = 133.0 nM 6.88
CHEMBL21859 Ki = 302.0 nM 6.52
CHEMBL281388 Ki = 1860.0 nM 5.73
CHEMBL281388 Ki = 1890.0 nM 5.72
CHEMBL428704 Ki = 2680.0 nM 5.57
CHEMBL283154 Ki = 4380.0 nM 5.36
CHEMBL283154 Ki = 5100.0 nM 5.29
CHEMBL428704 Ki = 7640.0 nM 5.12