Assay Detail
Binding
CHEMBL746305
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity against human muscarine receptor (hM4) cloned in CHO cells
10
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and affinity studies of himbacine derived muscarinic receptor antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 10 | 6.47 | 8.06 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL277642 | HIMBACINE | — | 2 | 8.06 |
| CHEMBL301670 | — | — | 1 | 6.76 |
| CHEMBL57485 | — | — | 1 | 6.21 |
| CHEMBL58824 | — | — | 1 | 6.10 |
| CHEMBL55698 | — | — | 1 | 6.05 |
| CHEMBL294663 | — | — | 1 | 6.05 |
| CHEMBL56275 | — | — | 1 | 6.00 |
| CHEMBL291562 | — | — | 1 | 5.76 |
| CHEMBL430587 | — | — | 1 | 5.71 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL277642 | HIMBACINE | Ki | = | 8.71 | nM | 8.06 |
| CHEMBL277642 | HIMBACINE | Ki | = | 10.96 | nM | 7.96 |
| CHEMBL301670 | — | Ki | = | 173.78 | nM | 6.76 |
| CHEMBL57485 | — | Ki | = | 616.6 | nM | 6.21 |
| CHEMBL58824 | — | Ki | = | 794.33 | nM | 6.10 |
| CHEMBL55698 | — | Ki | = | 891.25 | nM | 6.05 |
| CHEMBL294663 | — | Ki | = | 891.25 | nM | 6.05 |
| CHEMBL56275 | — | Ki | = | 1000.0 | nM | 6.00 |
| CHEMBL291562 | — | Ki | = | 1737.8 | nM | 5.76 |
| CHEMBL430587 | — | Ki | = | 1949.84 | nM | 5.71 |