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Assay Detail

CHEMBL747822

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of binding of [3H]quinuclidinyl benzilate to muscarinic receptors in membranes of CHO cells transfected with Muscarinic acetylcholine receptor M3
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

6beta-Acetoxynortropane: a potent muscarinic agonist with apparent selectivity toward M2-receptors.
Pei XF, Gupta TH, Badio B, Padgett WL, Daly JW.
J Med Chem (1998) 41 : 2047 -2055
PubMed 9622546 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 4 5.25 6.17

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL64000 1 6.17
CHEMBL7303 ARECOLINE -1.0 1 5.24
CHEMBL294273 1 4.82
CHEMBL64788 1 4.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL64000 Ki = 680.0 nM 6.17
CHEMBL7303 ARECOLINE Ki = 5700.0 nM 5.24
CHEMBL294273 Ki = 15000.0 nM 4.82
CHEMBL64788 Ki = 18000.0 nM 4.75