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Assay Detail

CHEMBL748034

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory concentration was evaluated by Inhibiting 50% of Neutral endopeptidase enzyme (NEP) activity using 20 nM [3H]D-Ala2-Leu-enkephalin as substrate
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Oryctolagus cuniculus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Neprilysin (CHEMBL3768)
Type SINGLE PROTEIN
Organism Oryctolagus cuniculus

Publication

Exploration of neutral endopeptidase active site by a series of new thiol-containing inhibitors.
Gomez-Monterrey I, Turcaud S, Lucas E, Bruetschy L, Roques BP, Fournié-Zaluski MC.
J Med Chem (1993) 36 : 87 -94
PubMed 8421293 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.40 8.44

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2110347 1 8.44
CHEMBL10247 THIORPHAN -1.0 1 8.40
CHEMBL55286 1 7.85
CHEMBL174237 1 7.70
CHEMBL171850 1 7.58
CHEMBL2110258 1 7.46
CHEMBL173287 1 7.43
CHEMBL173919 1 7.40
CHEMBL54601 1 7.36
CHEMBL174066 1 7.30
CHEMBL367174 1 7.30
CHEMBL366937 1 7.30
CHEMBL172787 1 7.28
CHEMBL175110 1 6.75
CHEMBL176960 1 6.50
CHEMBL172978 1 6.42

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2110347 IC50 = 3.6 nM 8.44
CHEMBL10247 THIORPHAN IC50 = 4.0 nM 8.40
CHEMBL55286 IC50 = 14.0 nM 7.85
CHEMBL174237 IC50 = 20.0 nM 7.70
CHEMBL171850 IC50 = 26.0 nM 7.58
CHEMBL2110258 IC50 = 35.0 nM 7.46
CHEMBL173287 IC50 = 37.0 nM 7.43
CHEMBL173919 IC50 = 40.0 nM 7.40
CHEMBL54601 IC50 = 44.0 nM 7.36
CHEMBL174066 IC50 = 50.0 nM 7.30
CHEMBL367174 IC50 = 50.0 nM 7.30
CHEMBL366937 IC50 = 50.0 nM 7.30
CHEMBL172787 IC50 = 53.0 nM 7.28
CHEMBL175110 IC50 = 180.0 nM 6.75
CHEMBL176960 IC50 = 320.0 nM 6.50
CHEMBL172978 IC50 = 380.0 nM 6.42