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Assay Detail

CHEMBL748247

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against [3H]quinuclidinyl Benzilate binding to Muscarinic acetylcholine receptor M2 in the absence of GTP
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

6beta-Acetoxynortropane: a potent muscarinic agonist with apparent selectivity toward M2-receptors.
Pei XF, Gupta TH, Badio B, Padgett WL, Daly JW.
J Med Chem (1998) 41 : 2047 -2055
PubMed 9622546 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.86 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL64000 1 7.77
CHEMBL14 CARBACHOL 4.0 1 6.89
CHEMBL978 METHACHOLINE 4.0 1 6.42
CHEMBL12587 MUSCARINE 1 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL64000 IC50 = 17.0 nM 7.77
CHEMBL14 CARBACHOL IC50 = 130.0 nM 6.89
CHEMBL978 METHACHOLINE IC50 = 380.0 nM 6.42
CHEMBL12587 MUSCARINE IC50 = 449.0 nM 6.35