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Assay Detail

CHEMBL748612

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Binding
In vitro receptor binding against Muscarinic acetylcholine receptor M3 in rat submandibular gland was determined using [3H]pirenzepine
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Submandibular gland
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M3 (CHEMBL320)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and biological evaluation of new antimuscarinic compounds with amidine basic centers. A useful bioisosteric replacement of classical cationic heads.
Cereda E, Ezhaya A, Gil Quintero M, Bellora E, Dubini E, Micheletti R, Schiavone A, Brambilla A, Schiavi GB, Donetti A.
J Med Chem (1990) 33 : 2108 -2113
PubMed 2374141 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 11 8.55 9.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL293927 1 9.36
CHEMBL517712 ATROPINE 4.0 1 9.20
CHEMBL569713 SCOPOLAMINE 4.0 1 9.19
CHEMBL553066 1 8.85
CHEMBL553894 1 8.60
CHEMBL2146143 ATROPINE METHYLBROMIDE -1.0 1 8.59
CHEMBL553783 1 8.51
CHEMBL544759 1 8.46
CHEMBL553562 1 8.35
CHEMBL544993 1 8.30
CHEMBL21823 1 6.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL293927 Kd = 0.44 nM 9.36
CHEMBL517712 ATROPINE Kd = 0.63 nM 9.20
CHEMBL569713 SCOPOLAMINE Kd = 0.65 nM 9.19
CHEMBL553066 Kd = 1.4 nM 8.85
CHEMBL553894 Kd = 2.5 nM 8.60
CHEMBL2146143 ATROPINE METHYLBROMIDE Kd = 2.6 nM 8.59
CHEMBL553783 Kd = 3.1 nM 8.51
CHEMBL544759 Kd = 3.5 nM 8.46
CHEMBL553562 Kd = 4.5 nM 8.35
CHEMBL544993 Kd = 5.0 nM 8.30
CHEMBL21823 Kd = 220.0 nM 6.66