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Assay Detail

CHEMBL748773

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
The compound was tested for antagonist activity by selective inhibition of [35S]GTP-gamma-S, binding to Opioid receptor mu 1 in Guinea pig caudate stimulated by DAMGO[mu]
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Cavia porcellus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Mu-type opioid receptor (CHEMBL4354)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Investigation of the N-substituent conformation governing potency and mu receptor subtype-selectivity in (+)-(3R, 4R)-dimethyl-4-(3-hydroxyphenyl)piperidine opioid antagonists.
Thomas JB, Mascarella SW, Rothman RB, Partilla JS, Xu H, McCullough KB, Dersch CM, Cantrell BE, Zimmerman DM, Carroll FI.
J Med Chem (1998) 41 : 1980 -1990
PubMed 9599247 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 5 10.32 10.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL52451 1 10.89
CHEMBL300662 1 10.68
CHEMBL50594 1 10.59
CHEMBL51838 1 10.41
CHEMBL19019 NALTREXONE 4.0 1 9.03

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL52451 Ki = 0.013 nM 10.89
CHEMBL300662 Ki = 0.021 nM 10.68
CHEMBL50594 Ki = 0.026 nM 10.59
CHEMBL51838 Ki = 0.039 nM 10.41
CHEMBL19019 NALTREXONE Ki = 0.93 nM 9.03