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Assay Detail

CHEMBL748904

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of binding of [3H]quinuclidinyl benzilate to muscarinic receptors in membranes of CHO cells transfected with Muscarinic acetylcholine receptor M2
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

6beta-Acetoxynortropane: a potent muscarinic agonist with apparent selectivity toward M2-receptors.
Pei XF, Gupta TH, Badio B, Padgett WL, Daly JW.
J Med Chem (1998) 41 : 2047 -2055
PubMed 9622546 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 7 7.13 8.59

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL64000 1 8.59
CHEMBL14 CARBACHOL 4.0 1 7.70
CHEMBL7303 ARECOLINE -1.0 1 7.40
CHEMBL978 METHACHOLINE 4.0 1 7.23
CHEMBL12587 MUSCARINE 1 7.12
CHEMBL64788 1 6.32
CHEMBL294273 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL64000 Ki = 2.6 nM 8.59
CHEMBL14 CARBACHOL Ki = 20.0 nM 7.70
CHEMBL7303 ARECOLINE Ki = 40.0 nM 7.40
CHEMBL978 METHACHOLINE Ki = 59.0 nM 7.23
CHEMBL12587 MUSCARINE Ki = 75.0 nM 7.12
CHEMBL64788 Ki = 480.0 nM 6.32
CHEMBL294273 Ki = 2700.0 nM 5.57