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Assay Detail

CHEMBL749021

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Tested for affinity constant against M1 muscarinic receptor in rat cortex using [3H]pirenzepine
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design, synthesis, and biological activity of methoctramine-related tetraamines bearing an 11-acetyl-5,11-dihydro-6H-pyrido[2,3-b][1,4] benzodiazepin-6-one moiety: structural requirements for optimum occupancy of muscarinic receptor subtypes as revealed by symmetrical and unsymmetrical polyamines.
Minarini A, Bolognesi ML, Budriesi R, Canossa M, Chiarini A, Spampinato S, Melchiorre C.
J Med Chem (1994) 37 : 3363 -3372
PubMed 7932564 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 8.12 8.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201994 1 8.90
CHEMBL442014 1 8.77
CHEMBL1201996 1 8.73
CHEMBL1201995 1 8.60
CHEMBL112244 1 8.57
CHEMBL1201998 1 8.32
CHEMBL9967 PIRENZEPINE 2.0 1 8.19
CHEMBL1201997 1 7.80
CHEMBL265256 TRIPITRAMINE 1 7.63
CHEMBL116172 1 7.55
CHEMBL27673 METHOCTRAMINE 1 7.43
CHEMBL43383 1 6.98

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201994 Ki = 1.259 nM 8.90
CHEMBL442014 Ki = 1.698 nM 8.77
CHEMBL1201996 Ki = 1.862 nM 8.73
CHEMBL1201995 Ki = 2.512 nM 8.60
CHEMBL112244 Ki = 2.692 nM 8.57
CHEMBL1201998 Ki = 4.786 nM 8.32
CHEMBL9967 PIRENZEPINE Ki = 6.457 nM 8.19
CHEMBL1201997 Ki = 15.85 nM 7.80
CHEMBL265256 TRIPITRAMINE Ki = 23.44 nM 7.63
CHEMBL116172 Ki = 28.18 nM 7.55
CHEMBL27673 METHOCTRAMINE Ki = 37.15 nM 7.43
CHEMBL43383 Ki = 104.71 nM 6.98