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Assay Detail

CHEMBL749270

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to inhibit binding of [3H]QNB to Muscarinic acetylcholine receptor in cultured N4TG1 neuroblastoma cells at 1e-8 to 1e-3 M
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Muscarinic acetylcholine receptor (CHEMBL2097162)
Type PROTEIN FAMILY
Organism Mus musculus

Publication

Inhibition of muscarinic receptor binding and acetylcholine-induced contraction of guinea pig ileum by analogues of 5'-(isobutylthio)adenosine.
Pankaskie MC, Kachur JF, Itoh T, Gordon RK, Chiang PK.
J Med Chem (1985) 28 : 1117 -1119
PubMed 3874962 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.35 4.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL604771 1 4.41
CHEMBL604770 1 4.28
CHEMBL13607 1 -
CHEMBL605813 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL604771 IC50 = 39000.0 nM 4.41
CHEMBL604770 IC50 = 52000.0 nM 4.28
CHEMBL13607 IC50 = 210000.0 nM -
CHEMBL605813 IC50 = 1500000.0 nM -