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Assay Detail

CHEMBL749444

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Growth of NIH3T3 cell colonies in soft agar with continuous treatment
6
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type NIH3T3
Confidence 1 — Organism
Curated By Expert

Target

NIH3T3 (CHEMBL614822)
Type CELL-LINE
Organism Mus musculus

Publication

2-Substituted aminopyrido[2,3-d]pyrimidin-7(8H)-ones. structure-activity relationships against selected tyrosine kinases and in vitro and in vivo anticancer activity.
Klutchko SR, Hamby JM, Boschelli DH, Wu Z, Kraker AJ, Amar AM, Hartl BG, Shen C, Klohs WD, Steinkampf RW, Driscoll DL, Nelson JM, Elliott WL, Roberts BJ, Stoner CL, Vincent PW, Dykes DJ, Panek RL, Lu GH, Major TC, Dahring TK, Hallak H, Bradford LA, Showalter HD, Doherty AM.
J Med Chem (1998) 41 : 3276 -3292
PubMed 9703473 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 7.89 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL49120 PD-0166285 1.0 1 8.96
CHEMBL112914 1 8.82
CHEMBL301612 2 8.82
CHEMBL297523 1 7.77
CHEMBL104779 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL49120 PD-0166285 IC50 = 1.1 nM 8.96
CHEMBL112914 IC50 = 1.5 nM 8.82
CHEMBL301612 IC50 = 1.5 nM 8.82
CHEMBL297523 IC50 = 17.0 nM 7.77
CHEMBL104779 IC50 = 306.0 nM 6.51
CHEMBL301612 IC50 = 370.0 nM 6.43