Assay Detail
Binding
CHEMBL749767
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Compound was evaluated for its binding affinity at human muscarinic receptor m4 by the displacement of [3H]NMS radioligand using membranes from transfected chinese hamster ovarian cell
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | ovarian |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Identification and characterization of m4 selective muscarinic antagonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 2 | 9.02 | 9.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 9.40 |
| CHEMBL376897 | METHSCOPOLAMINE | 4.0 | 1 | 8.64 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL14 | CARBACHOL | IC50 | = | 0.4 | nM | 9.40 |
| CHEMBL376897 | METHSCOPOLAMINE | IC50 | = | 2.3 | nM | 8.64 |