Assay Detail
Binding
CHEMBL749860
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Binding affinity (Ki) against binding of [3H]NMS using membranes from CHO cells expressing cloned human Muscarinic acetylcholine receptor M4
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Cyclohexylmethylpiperidinyltriphenylpropioamide: a selective muscarinic M(3) antagonist discriminating against the other receptor subtypes.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 10 | 7.87 | 9.47 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL517712 | ATROPINE | 4.0 | 1 | 9.47 |
| CHEMBL2112957 | — | — | 1 | 8.64 |
| CHEMBL1346 | DARIFENACIN | 4.0 | 1 | 8.07 |
| CHEMBL2112958 | — | — | 1 | 7.96 |
| CHEMBL2112955 | — | — | 1 | 7.85 |
| CHEMBL2112956 | — | — | 1 | 7.52 |
| CHEMBL2112959 | — | — | 1 | 7.51 |
| CHEMBL2112961 | — | — | 1 | 7.37 |
| CHEMBL369062 | — | — | 1 | 7.22 |
| CHEMBL177040 | — | — | 1 | 7.13 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL517712 | ATROPINE | Ki | = | 0.34 | nM | 9.47 |
| CHEMBL2112957 | — | Ki | = | 2.3 | nM | 8.64 |
| CHEMBL1346 | DARIFENACIN | Ki | = | 8.6 | nM | 8.07 |
| CHEMBL2112958 | — | Ki | = | 11.0 | nM | 7.96 |
| CHEMBL2112955 | — | Ki | = | 14.0 | nM | 7.85 |
| CHEMBL2112956 | — | Ki | = | 30.0 | nM | 7.52 |
| CHEMBL2112959 | — | Ki | = | 31.0 | nM | 7.51 |
| CHEMBL2112961 | — | Ki | = | 43.0 | nM | 7.37 |
| CHEMBL369062 | — | Ki | = | 60.0 | nM | 7.22 |
| CHEMBL177040 | — | Ki | = | 74.0 | nM | 7.13 |