Assay Detail
Binding
CHEMBL749864
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Inhibition of [3H]- quinuclidinyl benzilate binding to Muscarinic acetylcholine receptor M4 expressed in CHO cell membranes
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Expert |
Target
Muscarinic acetylcholine receptor M4 (CHEMBL1821) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
6beta-Acyloxy(nor)tropanes: affinities for antagonist/agonist binding sites on transfected and native muscarinic receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 14 | 6.36 | 7.48 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL64000 | — | — | 1 | 7.48 |
| CHEMBL314493 | — | — | 1 | 7.38 |
| CHEMBL315428 | — | — | 1 | 7.32 |
| CHEMBL7634 | OXOTREMORINE | — | 1 | 6.80 |
| CHEMBL84113 | — | — | 1 | 6.72 |
| CHEMBL313738 | — | — | 1 | 6.52 |
| CHEMBL85190 | — | — | 1 | 6.39 |
| CHEMBL310558 | — | — | 1 | 6.18 |
| CHEMBL82754 | — | — | 1 | 6.08 |
| CHEMBL440138 | — | — | 1 | 6.02 |
| CHEMBL12587 | MUSCARINE | — | 1 | 5.85 |
| CHEMBL7303 | ARECOLINE | -1.0 | 1 | 5.62 |
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 5.58 |
| CHEMBL294273 | — | — | 1 | 5.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL64000 | — | Ki | = | 33.0 | nM | 7.48 |
| CHEMBL314493 | — | Ki | = | 42.0 | nM | 7.38 |
| CHEMBL315428 | — | Ki | = | 48.0 | nM | 7.32 |
| CHEMBL7634 | OXOTREMORINE | Ki | = | 160.0 | nM | 6.80 |
| CHEMBL84113 | — | Ki | = | 190.0 | nM | 6.72 |
| CHEMBL313738 | — | Ki | = | 300.0 | nM | 6.52 |
| CHEMBL85190 | — | Ki | = | 410.0 | nM | 6.39 |
| CHEMBL310558 | — | Ki | = | 660.0 | nM | 6.18 |
| CHEMBL82754 | — | Ki | = | 840.0 | nM | 6.08 |
| CHEMBL440138 | — | Ki | = | 960.0 | nM | 6.02 |
| CHEMBL12587 | MUSCARINE | Ki | = | 1400.0 | nM | 5.85 |
| CHEMBL7303 | ARECOLINE | Ki | = | 2400.0 | nM | 5.62 |
| CHEMBL14 | CARBACHOL | Ki | = | 2600.0 | nM | 5.58 |
| CHEMBL294273 | — | Ki | = | 8300.0 | nM | 5.08 |