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Assay Detail

CHEMBL750255

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory potency of compound in presence of Mn2+ ion against NS5B HCV polymerase
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepacivirus hominis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RNA-directed RNA polymerase (CHEMBL4296320)
Type SINGLE PROTEIN
Organism Hepacivirus hominis

Publication

The monoethyl ester of meconic acid is an active site inhibitor of HCV NS5B RNA-dependent RNA polymerase.
Pace P, Nizi E, Pacini B, Pesci S, Matassa V, De Francesco R, Altamura S, Summa V.
Bioorg Med Chem Lett (2004) 14 : 3257 -3261
PubMed 15149686 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.43 6.19

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL109569 1 6.19
CHEMBL323046 1 6.10
CHEMBL110021 1 5.40
CHEMBL109516 1 4.75
CHEMBL326470 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL109569 IC50 = 650.0 nM 6.19
CHEMBL323046 IC50 = 800.0 nM 6.10
CHEMBL110021 IC50 = 4000.0 nM 5.40
CHEMBL109516 IC50 = 18000.0 nM 4.75
CHEMBL326470 IC50 = 19000.0 nM 4.72