Assay Detail
Binding
CHEMBL750358
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity towards rat forebrain nicotinic acetylcholine receptor using [3H]EB as radioligand
10
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Rattus norvegicus |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Target
Neuronal acetylcholine receptor (CHEMBL2094110) ↗| Type | PROTEIN COMPLEX GROUP |
| Organism | Rattus norvegicus |
Publication
Pharmacology of the agonist binding sites of rat neuronal nicotinic receptor subtypes expressed in HEK 293 cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 10 | 8.08 | 10.19 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL6623 | EPIBATIDINE | — | 2 | 10.19 |
| CHEMBL55138 | — | — | 1 | 9.85 |
| CHEMBL59986 | — | — | 1 | 9.66 |
| CHEMBL497939 | CYTISINICLINE | 3.0 | 1 | 8.60 |
| CHEMBL3 | NICOTINE | 4.0 | 1 | 7.89 |
| CHEMBL667 | ACETYLCHOLINE | 4.0 | 1 | 7.37 |
| CHEMBL47814 | DIMETHYLPHENYLPIPERAZINIUM IODIDE | — | 1 | 6.92 |
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 6.35 |
| CHEMBL920 | CHOLINE | 0.5 | 1 | 4.38 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL6623 | EPIBATIDINE | Ki | = | 0.064 | nM | 10.19 |
| CHEMBL55138 | — | Ki | = | 0.14 | nM | 9.85 |
| CHEMBL59986 | — | Ki | = | 0.22 | nM | 9.66 |
| CHEMBL6623 | EPIBATIDINE | Ki | = | 0.29 | nM | 9.54 |
| CHEMBL497939 | CYTISINICLINE | Ki | = | 2.5 | nM | 8.60 |
| CHEMBL3 | NICOTINE | Ki | = | 13.0 | nM | 7.89 |
| CHEMBL667 | ACETYLCHOLINE | Ki | = | 43.0 | nM | 7.37 |
| CHEMBL47814 | DIMETHYLPHENYLPIPERAZINIUM IODIDE | Ki | = | 120.0 | nM | 6.92 |
| CHEMBL14 | CARBACHOL | Ki | = | 450.0 | nM | 6.35 |
| CHEMBL920 | CHOLINE | Ki | = | 42000.0 | nM | 4.38 |