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Assay Detail

CHEMBL750359

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Compound was evaluated for its ability to displace [3H]-cytisine binding from high-affinity Nicotinic acetylcholine receptor in rat brain (principally the alpha4-beta2 nAChR subtype)
8
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Neuronal acetylcholine receptor (CHEMBL2094110)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Identification and initial structure-activity relationships of (R)-5-(2-azetidinylmethoxy)-2-chloropyridine (ABT-594), a potent, orally active, non-opiate analgesic agent acting via neuronal nicotinic acetylcholine receptors.
Holladay MW, Wasicak JT, Lin NH, He Y, Ryther KB, Bannon AW, Buckley MJ, Kim DJ, Decker MW, Anderson DJ, Campbell JE, Kuntzweiler TA, Donnelly-Roberts DL, Piattoni-Kaplan M, Briggs CA, Williams M, Arneric SP.
J Med Chem (1998) 41 : 407 -412
PubMed 9484491 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 9.87 10.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL430497 TEBANICLINE 2.0 2 10.40
CHEMBL6623 EPIBATIDINE 1 10.37
CHEMBL262769 1 10.30
CHEMBL334860 1 10.05
CHEMBL132486 1 9.35
CHEMBL59986 1 9.28
CHEMBL133570 1 8.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL430497 TEBANICLINE Ki = 0.04 nM 10.40
CHEMBL430497 TEBANICLINE Ki = 0.04 nM 10.40
CHEMBL6623 EPIBATIDINE Ki = 0.043 nM 10.37
CHEMBL262769 Ki = 0.05 nM 10.30
CHEMBL334860 Ki = 0.09 nM 10.05
CHEMBL132486 Ki = 0.45 nM 9.35
CHEMBL59986 Ki = 0.52 nM 9.28
CHEMBL133570 Ki = 1.6 nM 8.80