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Assay Detail

CHEMBL750473

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NMDA receptor binding affinity in rat brain synaptosomal membrane using [3H]glutamate as radioligand
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate NMDA receptor (CHEMBL1907608)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Potent, orally active, competitive N-methyl-D-aspartate (NMDA) receptor antagonists are substrates for a neutral amino acid uptake system in Chinese hamster ovary cells.
Li JH, Bigge CF, Williamson RM, Borosky SA, Vartanian MG, Ortwine DF.
J Med Chem (1995) 38 : 1955 -1965
PubMed 7783127 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 6.48 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL60181 1 7.85
CHEMBL62030 1 7.82
CHEMBL1907813 1 6.89
CHEMBL61650 1 6.50
CHEMBL294173 1 6.19
CHEMBL22304 1 6.08
CHEMBL59566 1 5.64
CHEMBL58365 1 4.87

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL60181 IC50 = 14.0 nM 7.85
CHEMBL62030 IC50 = 15.0 nM 7.82
CHEMBL1907813 IC50 = 130.0 nM 6.89
CHEMBL61650 IC50 = 320.0 nM 6.50
CHEMBL294173 IC50 = 650.0 nM 6.19
CHEMBL22304 IC50 = 837.0 nM 6.08
CHEMBL59566 IC50 = 2300.0 nM 5.64
CHEMBL58365 IC50 = 13500.0 nM 4.87