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Assay Detail

CHEMBL751067

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibitory activity against [3H]DAMGO binding to Opioid receptor mu 1 in rat brain membranes
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Brain
Subcellular Brain membranes
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Endomorphin-1 analogues containing beta-proline are mu-opioid receptor agonists and display enhanced enzymatic hydrolysis resistance.
Cardillo G, Gentilucci L, Qasem AR, Sgarzi F, Spampinato S.
J Med Chem (2002) 45 : 2571 -2578
PubMed 12036366 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.65 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL316446 ENDOMORPHIN-1 1 9.30
CHEMBL316447 1 8.40
CHEMBL38874 DAMGO 1 8.00
CHEMBL87907 1 7.63
CHEMBL313820 1 7.10
CHEMBL430643 1 7.10
CHEMBL2112324 1 6.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL316446 ENDOMORPHIN-1 IC50 = 0.5 nM 9.30
CHEMBL316447 IC50 = 4.0 nM 8.40
CHEMBL38874 DAMGO IC50 = 9.9 nM 8.00
CHEMBL87907 IC50 = 23.3 nM 7.63
CHEMBL313820 IC50 = 79.0 nM 7.10
CHEMBL430643 IC50 = 80.1 nM 7.10
CHEMBL2112324 IC50 = 990.0 nM 6.00