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Assay Detail

CHEMBL751235

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Tested in vitro for antagonistic activity at N-methyl-D-aspartate glutamate receptor by measuring displacement of [3H]TCP from crude synaptic membrane preparations obtained from rat brain
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate NMDA receptor (CHEMBL1907608)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Design and synthesis of [2-(8,9-dioxo-2,6-diazabicyclo[5.2.0]non-1(7)-en-2-yl)-ethyl]phosphonic acid (EAA-090), a potent N-methyl-D-aspartate antagonist, via the use of 3-cyclobutene-1,2-dione as an achiral alpha-amino acid bioisostere.
Kinney WA, Abou-Gharbia M, Garrison DT, Schmid J, Kowal DM, Bramlett DR, Miller TL, Tasse RP, Zaleska MM, Moyer JA.
J Med Chem (1998) 41 : 236 -246
PubMed 9457246 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.75 8.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL452461 PERZINFOTEL 2.0 1 8.12
CHEMBL79314 1 8.00
CHEMBL79960 1 7.75
CHEMBL39664 SELFOTEL 2.0 1 7.66
CHEMBL309544 1 7.54
CHEMBL310445 1 7.44
CHEMBL81836 1 -
CHEMBL79949 1 -
CHEMBL415691 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL452461 PERZINFOTEL IC50 = 7.6 nM 8.12
CHEMBL79314 IC50 = 10.0 nM 8.00
CHEMBL79960 IC50 = 18.0 nM 7.75
CHEMBL39664 SELFOTEL IC50 = 22.0 nM 7.66
CHEMBL309544 IC50 = 29.0 nM 7.54
CHEMBL310445 IC50 = 36.0 nM 7.44
CHEMBL81836 IC50 > 100.0 nM -
CHEMBL79949 IC50 > 100.0 nM -
CHEMBL415691 IC50 > 100.0 nM -