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Assay Detail

CHEMBL751449

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]nisoxetine from norepinephrine transporter (NET) in rat brain membranes
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Brain membranes
Confidence 9 — Direct single protein target
Curated By Expert

Target

Norepinephrine transporter (CHEMBL304)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and ligand binding of nortropane derivatives: N-substituted 2beta-carbomethoxy-3beta-(4'-iodophenyl)nortropane and N-(3-iodoprop-(2E)-enyl)-2beta-carbomethoxy-3beta-(3',4'-disubstituted phenyl)nortropane. New high-affinity and selective compounds for the dopamine transporter.
Emond P, Garreau L, Chalon S, Boazi M, Caillet M, Bricard J, Frangin Y, Mauclaire L, Besnard JC, Guilloteau D.
J Med Chem (1997) 40 : 1366 -1372
PubMed 9135033 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 12 6.45 7.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL311347 1 7.10
CHEMBL611180 1 6.53
CHEMBL611426 1 6.40
CHEMBL2113650 1 6.30
CHEMBL2113649 1 6.22
CHEMBL611179 1 6.12
CHEMBL611177 1 -
CHEMBL611178 1 -
CHEMBL2113648 1 -
CHEMBL611424 1 -
CHEMBL611181 1 -
CHEMBL611427 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL311347 Ki = 80.0 nM 7.10
CHEMBL611180 Ki = 295.0 nM 6.53
CHEMBL611426 Ki = 400.0 nM 6.40
CHEMBL2113650 Ki = 500.0 nM 6.30
CHEMBL2113649 Ki = 600.0 nM 6.22
CHEMBL611179 Ki = 750.0 nM 6.12
CHEMBL611177 Ki > 1000.0 nM -
CHEMBL611178 Ki > 1000.0 nM -
CHEMBL2113648 Ki > 1000.0 nM -
CHEMBL611424 Ki > 1000.0 nM -
CHEMBL611181 Ki > 1000.0 nM -
CHEMBL611427 Ki > 1000.0 nM -