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Assay Detail

CHEMBL751540

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
In vitro binding affinity towards Muscarinic acetylcholine receptor M1 by the displacement of [3H]pirenzepine in rat cerebral cortical membranes
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Subcellular Membrane
Confidence 8 — Homologous single protein target
Curated By Expert

Target

Muscarinic acetylcholine receptor M1 (CHEMBL276)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis and structure activity relationships of alkyl substituted analogues of the functional M1 selective muscarinic receptor agonist xanomeline
Quimby SJ, Shannon HE, Bymaster FP, Sauerberg P, Olesen PH, Sheardown MJ, Suzdak PD, Mitch CH
Bioorg Med Chem Lett (1994) 4 : 2205 -2210
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.49 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL414874 1 8.30
CHEMBL69317 1 8.30
CHEMBL68160 1 8.30
CHEMBL21536 XANOMELINE 4.0 1 8.15
CHEMBL70051 1 8.15
CHEMBL70670 1 8.10
CHEMBL71536 1 8.10
CHEMBL305412 1 7.82
CHEMBL303922 1 7.75
CHEMBL303599 1 7.62
CHEMBL68482 1 7.32
CHEMBL70160 1 6.95
CHEMBL305401 1 6.83
CHEMBL302515 1 6.24
CHEMBL303732 1 6.00
CHEMBL71380 1 5.91

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL414874 IC50 = 5.0 nM 8.30
CHEMBL69317 IC50 = 5.0 nM 8.30
CHEMBL68160 IC50 = 5.0 nM 8.30
CHEMBL21536 XANOMELINE IC50 = 7.0 nM 8.15
CHEMBL70051 IC50 = 7.0 nM 8.15
CHEMBL70670 IC50 = 8.0 nM 8.10
CHEMBL71536 IC50 = 8.0 nM 8.10
CHEMBL305412 IC50 = 15.0 nM 7.82
CHEMBL303922 IC50 = 18.0 nM 7.75
CHEMBL303599 IC50 = 24.0 nM 7.62
CHEMBL68482 IC50 = 48.0 nM 7.32
CHEMBL70160 IC50 = 112.0 nM 6.95
CHEMBL305401 IC50 = 148.0 nM 6.83
CHEMBL302515 IC50 = 580.0 nM 6.24
CHEMBL303732 IC50 = 1000.0 nM 6.00
CHEMBL71380 IC50 = 1230.0 nM 5.91