Assay Detail
Binding
CHEMBL752093
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity of compound towards Nucleoside transporter es-type by facile competitive binding flow cytometric assay using the human K562 chronic melogenous leukemia cell line
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | K562 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Equilibrative nucleoside transporter 1 (CHEMBL1997) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Synthesis and flow cytometric evaluation of novel 1,2,3,4-tetrahydroisoquinoline conformationally constrained analogues of nitrobenzylmercaptopurine riboside (NBMPR) designed for probing its conformation when bound to the es nucleoside transporter.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 6 | 7.71 | 9.35 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL260570 | — | — | 1 | 9.35 |
| CHEMBL418509 | NITROBENZYLMERCAPTOPURINE RIBONUCLEOSIDE | — | 1 | 9.15 |
| CHEMBL261227 | — | — | 1 | 7.82 |
| CHEMBL429511 | — | — | 1 | 6.82 |
| CHEMBL408670 | — | — | 1 | 6.60 |
| CHEMBL261226 | — | — | 1 | 6.52 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL260570 | — | Ki | = | 0.45 | nM | 9.35 |
| CHEMBL418509 | NITROBENZYLMERCAPTOPURINE RIBONUCLEOSIDE | Ki | = | 0.7 | nM | 9.15 |
| CHEMBL261227 | — | Ki | = | 15.0 | nM | 7.82 |
| CHEMBL429511 | — | Ki | = | 150.0 | nM | 6.82 |
| CHEMBL408670 | — | Ki | = | 250.0 | nM | 6.60 |
| CHEMBL261226 | — | Ki | = | 300.0 | nM | 6.52 |