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Assay Detail

CHEMBL752855

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Ability to enhance [3H]MK-801 binding to NMDA receptors in rat forebrain membranes was calculated with respect to spermine
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Tissue Forebrain
Subcellular Membrane
Confidence 4 — Cell-line / Tissue
Curated By Autocuration

Target

Glutamate NMDA receptor (CHEMBL1907608)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis of N,N'-substituted piperazine and homopiperazine derivatives with polyamine-like actions at N-methyl-D-aspartate receptors.
Zhou LM, He XS, Li G, de Costa BR, Skolnick P.
J Med Chem (1995) 38 : 4891 -4896
PubMed 8523402 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 4.44 5.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL23194 SPERMINE 0.5 1 5.28
CHEMBL145680 1 4.75
CHEMBL144699 1 4.67
CHEMBL143613 1 4.61
CHEMBL147313 1 4.45
CHEMBL144094 1 4.43
CHEMBL144873 1 4.43
CHEMBL144518 1 4.34
CHEMBL41307 1 4.30
CHEMBL144067 1 4.29
CHEMBL144822 1 4.25
CHEMBL144255 1 4.19
CHEMBL144520 1 4.11
CHEMBL415535 1 4.01
CHEMBL144354 1 -
CHEMBL142725 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL23194 SPERMINE EC50 = 5200.0 nM 5.28
CHEMBL145680 EC50 = 18000.0 nM 4.75
CHEMBL144699 EC50 = 21400.0 nM 4.67
CHEMBL143613 EC50 = 24400.0 nM 4.61
CHEMBL147313 EC50 = 35300.0 nM 4.45
CHEMBL144094 EC50 = 36800.0 nM 4.43
CHEMBL144873 EC50 = 37500.0 nM 4.43
CHEMBL144518 EC50 = 45900.0 nM 4.34
CHEMBL41307 EC50 = 50100.0 nM 4.30
CHEMBL144067 EC50 = 50700.0 nM 4.29
CHEMBL144822 EC50 = 55900.0 nM 4.25
CHEMBL144255 EC50 = 64500.0 nM 4.19
CHEMBL144520 EC50 = 77500.0 nM 4.11
CHEMBL415535 EC50 = 96500.0 nM 4.01
CHEMBL144354 EC50 = 238500.0 nM -
CHEMBL142725 EC50 = 112200.0 nM -