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Assay Detail

CHEMBL753124

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]-MK-801 binding to NMDA receptor from rat cerebral cortical synaptic membrane
15
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Glutamate NMDA receptor (CHEMBL1907608)
Type PROTEIN COMPLEX GROUP
Organism Rattus norvegicus

Publication

Synthesis and biological activity of conformationally restricted analogs of milnacipran: (1S,2R)-1-phenyl-2-[(S)-1-aminopropyl]-N,N-diethylcyclopropanecarboxami de, an efficient noncompetitive N-methyl-D-aspartic acid receptor antagonist.
Shuto S, Ono S, Hase Y, Ueno Y, Noguchi T, Yoshii K, Matsuda A.
J Med Chem (1996) 39 : 4844 -4852
PubMed 8941398 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.67 8.01

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL275528 PHENCYCLIDINE 2.0 1 8.01
CHEMBL434836 1 6.80
CHEMBL119554 2 6.70
CHEMBL330958 2 6.46
CHEMBL742 KETAMINE 4.0 1 6.21
CHEMBL343694 1 6.00
CHEMBL145378 1 5.38
CHEMBL99946 LEVOMILNACIPRAN 4.0 1 5.20
CHEMBL356782 2 5.09
CHEMBL145226 1 4.77
CHEMBL145773 2 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL275528 PHENCYCLIDINE IC50 = 9.8 nM 8.01
CHEMBL434836 IC50 = 160.0 nM 6.80
CHEMBL119554 IC50 = 200.0 nM 6.70
CHEMBL330958 IC50 = 350.0 nM 6.46
CHEMBL742 KETAMINE IC50 = 610.0 nM 6.21
CHEMBL343694 IC50 = 1000.0 nM 6.00
CHEMBL145378 IC50 = 4200.0 nM 5.38
CHEMBL99946 LEVOMILNACIPRAN IC50 = 6300.0 nM 5.20
CHEMBL330958 IC50 = 6500.0 nM 5.19
CHEMBL119554 IC50 = 8200.0 nM 5.09
CHEMBL356782 IC50 = 8200.0 nM 5.09
CHEMBL356782 IC50 = 11000.0 nM 4.96
CHEMBL145226 IC50 = 17000.0 nM 4.77
CHEMBL145773 IC50 = 19000.0 nM 4.72
CHEMBL145773 IC50 = 31000.0 nM 4.51