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Assay Detail

CHEMBL753519

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity against opioid receptor kappa, in guinea pig brain membrane, using [3H]bremazocine as the radioligand.
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Cavia porcellus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Kappa-type opioid receptor (CHEMBL3952)
Type SINGLE PROTEIN
Organism Cavia porcellus

Publication

Structure/activity studies related to 2-(3,4-dichlorophenyl)-N-methyl-N-[2-(1-pyrrolidinyl)-1-substituted- ethyl]acetamides: a novel series of potent and selective kappa-opioid agonists.
Barlow JJ, Blackburn TP, Costello GF, James R, Le Count DJ, Main BG, Pearce RJ, Russell K, Shaw JS.
J Med Chem (1991) 34 : 3149 -3158
PubMed 1659636 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.25 8.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL38576 ICI-199,441 1 8.16
CHEMBL56653 1 8.16
CHEMBL105942 1 8.00
CHEMBL109205 1 7.97
CHEMBL320882 1 7.91
CHEMBL321061 1 7.85
CHEMBL106471 1 7.61
CHEMBL105984 1 7.41
CHEMBL56651 1 7.28
CHEMBL110924 1 7.24
CHEMBL105783 1 7.18
CHEMBL290543 1 7.02
CHEMBL106510 1 7.00
CHEMBL420115 1 5.99
CHEMBL70 MORPHINE 4.0 1 5.62
CHEMBL106345 1 5.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL38576 ICI-199,441 IC50 = 6.9 nM 8.16
CHEMBL56653 IC50 = 6.9 nM 8.16
CHEMBL105942 IC50 = 10.1 nM 8.00
CHEMBL109205 IC50 = 10.8 nM 7.97
CHEMBL320882 IC50 = 12.2 nM 7.91
CHEMBL321061 IC50 = 14.0 nM 7.85
CHEMBL106471 IC50 = 24.5 nM 7.61
CHEMBL105984 IC50 = 38.7 nM 7.41
CHEMBL56651 IC50 = 52.5 nM 7.28
CHEMBL110924 IC50 = 57.8 nM 7.24
CHEMBL105783 IC50 = 65.4 nM 7.18
CHEMBL290543 IC50 = 95.5 nM 7.02
CHEMBL106510 IC50 = 99.0 nM 7.00
CHEMBL420115 IC50 = 1020.0 nM 5.99
CHEMBL70 MORPHINE IC50 = 2390.0 nM 5.62
CHEMBL106345 IC50 = 2750.0 nM 5.56